Facilities and Equipment

Roden Geomicrobiology Laboratory | Xu Mineralogy/Nanogeoscience Laboratory | Other Geology & Geophysics Instrumentation | Environmental Chemistry and Technology Instrumentation | Biotechnology Center | Materials Research Science & Engineering Center | Microscopy Facilities

 

Roden Geomicrobiology Laboratory

I occupy a 1800 square foot laboratory in a new wing of Weeks Hall that houses the Department of Geology and Geophysics at UW-Madison. The laboratory is specifically equipped for handling anaerobic materials and microorganisms, in particular the culturing and manipulation of anaerobic respiratory and lithotrophic bacteria. The new laboratory also includes a molecular biological facility for nucleic acid extraction (from cultures or soil/sediment samples) and manipulation en route to sequencing and/or quantitative PCR analysis. Through ongoing DOE-funded (EMSP and NABIR programs; see Research Projects) research, a sediment core reactor facility for conducing reactive transport experiments with repacked and intact subsurface sediment cores has been developed. A diverse array of column reactors and associated pumping systems are available to support reactive transport studies involving microbial metabolism.

Major pieces of equipment available in my laboratory are as follows:

Autoclave (Primus)

Lyophilizer (Fisher Scientific)

Analytical balances (Denver Instruments)

UV/VIS spectrophotometer (Shimadzu)

Gas chromatograph with FID and TCD detectors (Shimadzu)

High-pressure liquid chromatograph with UV/VIS and RI detectors (Shimadzu)

Ion chromatograph (Dionex DX-100)

BET Surface area analyzer (Micromeritics)

Anaerobic chambers (Coy and Labconco)

High speed centrifuge (Sorvall RC5)

Microcentrifuges (Fisher)

Thermal cycler (BioRad/MJ DNA Engine)

Gel documentation system (Biorad)

DGGE system (BioRad D-Code)

Real-time PCR detector (BioRad/MJ Chromo 4)

Compound phase contrast/epifluorescence microscope (Nikon E600)

Temperature-regulated incubators

 

Xu (Huifang) Mineralogy/Nanogeoscience Laboratory

Xu occupies a laboratory adjacent to Roden in the new wing of the Geology and Geophysics. This laboratory is equipped for a variety of microscopic and spectroscopic mineralogical analyses, including:

X-ray diffraction (XRD): A Sintag Pad V automated powder XRD unit with Jade (Materials Data, Inc.) and Shaddow software for data reduction and analysis.

Microporosimeter: a new Micromeritics ASAP 2020 porosimeter that can measure BET surface area, nanopore surface area, and nanopore sizes will be installed in spring of 2005.

Atomic absorption spectrometer (AA): A Perkin-Elmer 5100PC atomic absorption spectrophotometer will be used for the concentration of metal ions in solutions.

Surface analysis system: A VCA Optima Surface Analysis System from AST Products that can measure static and dynamic contact angle and surface energy will be installed in laboratory in Spring of 2005.

Optical microscope: Olympus BX51

Instruments at the Materials Science Center

Transmission Electron Microscopes:

200 kV high-resolution Phillips CM200 Ultratwin TEM with 0.19 nm point-to-point resolution, Ge light element energy-dispersive X-ray spectroscopy (EDS) system, Gatan multi-scan CCD (charge-coupled device) camera, and full processing and analysis capabilities. NORAN Voyager 127 eV Be thin window EDS

A 120 kV energy-filtered TEM (LEO EM 912) with integrated omega filter that provides TEM imaging, electron energy-filtering TEM imaging, and electron diffraction from nanometer scale areas.

Vacuum Generators HB-501 ultra-high vacuum, field-emission gun STEM (for Z-contrast imaging) with light element EDS and parallel electron energy loss spectroscopy (PEELS)

Scanning Electron Microscopes:

A fully digital LEO GEMINI 1530 SEM with field emission electron gun, full orientation imaging based on backscattered electron Kikuchi patterns, and full EDS and control

A JSM-6100 is a modern Scanning Electron Microscope (SEM) that uses a tungsten thermionic electron source. It is an excellent general purpose SEM. The SEM is equipped with an onboard image averaging and acquisition system that greatly improves operation under difficult imaging conditions. In addition, there is a PC based active image acquisition system that allows the capture, processing, and electronic storage of high-resolution images. Other accessories include a Backscattered Electron Detector (BSE), and a full-featured PC based X-ray Energy-dispersive Analysis System (EDS).

A NORAN Instruments ADEM 30kV SEM with light element EDS and sophisticated image processing and feature analysis capabilities. The instrument has a 6-axis stage that can handle unusually large samples up to 8-inch diameter.

Focused Ion Beam (FIB): A newly installed Zeiss 1540 XBeam FESEM/FIB with in-lens secondary and backscattered detectors, gas injectors for deposition and etching, and micromanipulators for TEM sample lift-out.

XRD: Stoe X-ray diffractometer. The Stoe Instrument is a High Resolution X-ray Diffractometer. 2-theta range: 0.15 degrees to 140 degrees.

PANalytical X’Pert PRO MRD system: (high-resolution X-ray diffraction) can be configured with a hybrid monochromator or a high-resolution monochromator to fulfill high-resolution X-ray diffraction requirements. With PANalytical’s X’Pert PRO Extended MRD system, an X-ray mirror and a high-resolution monochromator can be placed in line to deliver an incident X-ray beam that is not only highly monochromatic with a low divergence, but also has a high intensity. This high intensity is used to uncover the weakest details in a diffraction experiment. X’Pert Epitaxy and Smoothfit provides functionality to analyze rocking curves, reciprocal space maps and wafer maps. Rocking curves can be simulated and fitted using patented algorithms

 

Other instrumentation in Geology and Geophysics

MC-ICP-MS: The GV Instruments IsoProbe installed at UW-Madison is a next-generation multi-collector inductively-coupled plasma mass spectrometer (MC-ICP-MS) that was installed in 2000. The IsoProbe is capable of very high-precision isotopic analysis and trace metal analysis of a variety of geologically important elements including Li, B, Mg, S, Cl, Ca, Cr, Fe, Ni, Cu, Zn, Se, Sr, Nd, Hf, Os, Hg, Pb, Th, and U.

Microprobe: A Cameca SX-50/51 electron microprobe that was installed in 1993 is capable in-situ chemical composition, X-ray maps from micro-scale areas, back-scattering imaging, and X-ray EDS analysis and mapping. The lab is managed by a full-time 100% university-supported research scientist, Dr. John Fournelle.

Ion probe: A new multicollector Cameca large radius ion microprobe (funded by NSF) was installed in the Department of Geology and Geophysics, UW-Madison in early 2005 as a national facility for stable isotope studies (WiscSIMS). The ion probe can also provide spatial-resolved trace element analysis. The lab is managed by a full-time research scientist, Dr. Noriko Kita.

Thin section shop: A ¾-time thin section preparatory, Brian Hess, maintains a complete facility in the department for variety of thin and polished sections.

Machine shop: A well-equipped shop is managed by a half-time machinist, Lee Putman, who has made components used in many designed instruments in the department.

Electronics shop: Located in the department, used primary for geological instrumentation, development and repair. It is staffed by full-time electronics engineers Lee Powell and Neal Lord.

Instrumentation available in the Environmental Chemistry and Technology Program:

Inductively Coupled Plasma Optical Emission Spectrometer (PE Optima 4300 DV)
Electrophoresis system (PenKem 3000)
Particle counter/sizers (Brinkman time of transition analyzer and Brookhaven BI-2030-AT)
Inductively Coupled Plasma Mass Spectrometer (VG PlasmaQuad II+)
Voltammetry System (Radiometer Trace Lab 50 /HDME/SMDE/DME and 6mm RDE)
High Performance Liquid Chromatography System (Waters 600 with 991 diode array); Fourier Transform Infrared Spectrometer (Nicolet 60SX with 680DSP)
Carbon analyzers (Shimadzu TOC-5000 with SSM)
Trace Sulfur analyzer (APS instruments)

 

Instrumentation available in the Environmental Chemistry and Technology Program

Inductively Coupled Plasma Optical Emission Spectrometer (PE Optima 4300 DV)

Electrophoresis system (PenKem 3000)

Particle counter/sizers (Brinkman time of transition analyzer and Brookhaven BI-2030-AT)

Inductively Coupled Plasma Mass Spectrometer (VG PlasmaQuad II+)

Voltammetry System (Radiometer Trace Lab 50 /HDME/SMDE/DME and 6mm RDE)

High Performance Liquid Chromatography System (Waters 600 with 991 diode array); Fourier Transform Infrared Spectrometer (Nicolet 60SX with 680DSP)

Carbon analyzers (Shimadzu TOC-5000 with SSM)

Trace Sulfur analyzer (APS instruments)